PMC

Ultrastructure of Curosurf, meconium, and dextran. Transmission electron micrographs from Curosurf preparations after incubation with 0.02 mg/ml meconium (A), 0.3 mg/ml meconium (B), 0.3 mg/ml meconium + 5 mg/ml dextran (C), and 0.3 mg/ml meconium + 10 mg/ml dextran (D). With increasing concentrations of meconium, more unilamellar vesicles become visible (A and B). The flocculent material visible in the center of B most likely represents meconium material (arrow). The addition of increasing concentrations of dextran leads to more lamellar body like forms and less unilamellar vesicles (C and D).

(A) Representative chromatogram of paclitaxel and its metabolites by isotope dilution-LC-HRMS in meconium. (B) Pg per mg wet weight of meconium for paclitaxel (Pac), 3’-p-hydroxypaclitaxel (3-OH-Pac), and 6α-hydroxypaclitaxel (6-OH-Pac) in 8 meconium samples. The mean of three averaged replicates per individual is shown with the standard deviation. (C) Pg per mg wet weight of meconium plotted by days of chemotherapy received before delivery.

Recovery of steroids from meconium. (A) Analyte recovery from pooled meconium was tested via extraction from pooled meconium (white), dichloromethane stripped meconium (solid black), re-extraction of pooled meconium (vertical stripe) and as a negative control double charcoal stripped serum (cross-hatch). (B) Quantitative recovery from control meconium spiked with 5000 pg DHEA (white), 1000 pg AD (cross-hatch), and 1000 pg T (solid black). N = 3 for all experimental groups. SEM, standard error of measurement.

A. Classification of the microbiota at the Phylum level. Samples BM62-BM140 are neonate meconium and 7 samples are from healthy adults. The total reads for each sample are normalized to 2000. Neonate meconium samples are grouped by maternal clinical status: Healthy, subclinical, GDM (gestational diabetes) and DM (type 2 diabetes). B. Average taxa abundances between the neonate meconium and adult stool samples. HC group combined healthy and subclinical samples. C. Alpha rare plot shows the average microbiota diversity of the neonate meconium grouped by maternal status (HC, GDM and DM) and adult stool samples.